Examples of a lymphocyte cell nucleus (A-C) and a metaphase spread (D-F) after simultaneous labeling of the centromeric region of chromosome 9 with PNA COMBO-FISH probes.(A, D) green image plane: OregonGreen 488 ® (λ ex/em = 496/524 nm).(B, E) red image plane: TexasRed ® (λ ex/em = 595/615 nm).(C, F) The merged images indicate a correct colocalization of the PNA signals. Methods: COMBO-FISH uses either a set of oligo-nucleotide probes (15 - 25 mers) uniquely co-localizing at the target site or single oligo-stretches repetitively but exclusively binding to the target. These are designed by systematic sequence data base searches. COMBO-FISH probes form Hoogsteen or Watson-Crick bonds and protocols with or In contrast to standard FISH techniques, COMBO-FISH makes use of a bioinformatic search in sequence databases for probe design, so that it can be done for any species so far sequenced. In the original approach, oligonucleotide stretches of typical lengths of 15-30 nucleotides were selected in such a way that they only co-localize at the given Here's the list of 10 Best freshwater aquarium fish combinations along with tank size and recommended number of fish for each combination.
Avoid incompatible species and serious trouble with this list. You'll need a big tank for this combo, at least 30 gallons. Both species will eat anything you give them, from flakes to pellets to frozen
COMBO-FISH allows precise and focused fluorescent labeling of chromatin domains in the nucleus by a combination of computer-selected fluorescently labeled short DNA or PNA probes. This co-localization probe set hybridizes to a defined genomic region and generates a locus-specific fluorescent signal, which is specifically labeled about 50-250 kb COMBO-FISH takes advantage of homopurine/homopyrimidine oligonucleotides that form triple helices with intact duplex genomic DNA, without the need for prior denaturation of the target sequence that is usually applied for probe binding in standard FISH protocols. An analysis of human genome databases has shown that homopurine/homopyrimidine COMBO-FISH has been designed for DNA double and triplex [40] strands. In the latter case, special triples of bases are necessary which are able to form base triplets (Hoogsteen triplets). In its classical configuration, the third strand, intercalating into the major groove of the double strand, consists solely either of purines or pyrimidines COMBO-FISH: principle and applications. In contrast to standard FISH where probes are usually cut and amplified by molecular biology techniques, COMBinatorial Oligonucleotide FISH (COMBO-FISH) follows a completely different strategy for probe design [19, 20, 26, 27].If a genome of a species is sequenced and the sequence is cataloged in a database, oligonucleotide probes can be searched andCombinatorial Oligonucleotide FISH (COMBO-FISH): Computer Designed
COMBO-FISH allows precise and focused fluorescent labeling of chromatin domains in the nucleus by a combination of computer-selected fluorescently labeled short DNA or PNA probes. This co-localization probe set hybridizes to a defined genomic region and generates a locus-specific fluorescent signal, which is specifically labeled about 50-250 kb COMBO-FISH takes advantage of homopurine/homopyrimidine oligonucleotides that form triple helices with intact duplex genomic DNA, without the need for prior denaturation of the target sequence that is usually applied for probe binding in standard FISH protocols. An analysis of human genome databases has shown that homopurine/homopyrimidine COMBO-FISH has been designed for DNA double and triplex [40] strands. In the latter case, special triples of bases are necessary which are able to form base triplets (Hoogsteen triplets). In its classical configuration, the third strand, intercalating into the major groove of the double strand, consists solely either of purines or pyrimidines COMBO-FISH: principle and applications.
In contrast to standard FISH where probes are usually cut and amplified by molecular biology techniques, COMBinatorial Oligonucleotide FISH (COMBO-FISH) follows a completely different strategy for probe design [19, 20, 26, 27].If a genome of a species is sequenced and the sequence is cataloged in a database, oligonucleotide probes can be searched and
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